But the former gunner Olivier Giroud was dismissed by Didier Deschamps.
The Blues will travel to Italy next week with the hosts Spain and Belgium.
But they will travel without AC Milan striker Giroud, 35, top scorer in the history of his country.
This is the second time the ex-Arsenal and Chelsea man has been ignored after being ruled out of France’s World Cup qualifiers last month.
France will face Belgium in the semi-final on Thursday at Juventus stadium.
Lucas Hernandez and Benjamin Pavard are back in the squad with their Bayern Munich teammate Dayot Upamecano.
Guendouzi was called up as Chelsea’s N’Golo Kante is out after testing positive for Covid.
The entire French team
It is the team of 23 players that Didier Deschamps has chosen for the last four of the Nations League this month in Italy …
Defenders: Lucas Digne (Everton), Leo Dubois (Olympique Lyonnais), Lucas Hernandez (Bayern Munich), Presnel Kimpembe (Paris St Germain), Jules Kounde (Sevilla), Benjamin Pavard (Bayern Munich), Dayot Upamecano (Bayern Munich), Raphael Varane (Manchester United).
Midfielder: Matteo Guendouzi (Olympique de Marseille), Theo Hernandez (AC Milan), Paul Pogba (Manchester United), Adrien Rabiot (Juventus), Aurelien Tchouameni (Monaco), Jordan Veretout (Roma).
Before: Wissam Ben Yedder (Monaco), Karim Benzema (Real Madrid), Moussa Diaby (Bayer Leverkusen), Antoine Griezmann (Atletico Madrid), Anthony Martial (Manchester United), Kylian Mbappe (Paris St Germain).
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Deschamps said: “There is a 10 day isolation period so he couldn’t have trained and he was also just coming off an injury layoff.
“It’s better for him that he uses this period to recover his physical freshness. “
Guendouzi joined Marseille on loan from Arsenal this summer, but the bad boy is said to be falling out with his new teammates.
The 22-year-old has reportedly faced a number of the squad’s players, according to L’Equipe.
He got off to a good start for his new team, scoring once and providing an assist in his first three games.
But now Marseille have won just one of their last four games, much to the chagrin of the Arsenal lender.
In the 3-2 home loss to Lens this weekend, L’Equipe claim that Guendouzi asked his team-mate Gerson “when he was going to start running”, which did not go well.
‘FALL ON YOUR KNEES AND CRIES TO THE SKY’
Gerson had to be restrained by his teammates as Guendouzi walked away, with captain Dimitri Payet stepping in to try to calm him down.
The incident came after Lens’s second goal, with Guendouzi running towards the Brazilian before facing him and accusing him of not running.
And the French talk show Le Phoceen also reports the discord in the Marseille camp.
They claim that Guendouzi has been very critical of his teammates, and also incredibly dramatic.
It is even alleged that he fell to his knees and cried out to heaven when someone makes a bad first contact.
The Frenchman is also reported to moan and blame others when things don’t go as planned.
Guendouzi played 82 times during his time at Arsenal but had a reputation at the club for being a tough person to deal with.
There was a rift with club manager Mikel Arteta, and he was kicked out of the club after taunting Brighton players about making more money than them in 2020.
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The midfielder was also caught on camera in the same game grabbing Brighton match winner Neal Maupay by the throat and throwing him to the ground.
Guendouzi was then loaned to Hertha Berlin last season, then joined Marseille for this campaign.
The Ligue 1 side have an obligation to make the loan permanent for £ 9.4million at the end of the season.
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