The doctoral students, 2020-21 public researcher Hamid Orimi, and his co-authors present the feasibility of the new bioprinting technology they developed in a recent treatise published in the journal Micromachines. They explain how the methodology they created, called Laser-Induced Side Transfer (LIST), improves existing bioprinting techniques by using bioinks of different viscosities and enables better 3D printing. Is shown. Orimi co-authors, Concordia co-director Sivakumar Narayanswamy from Gina Cody School of Engineering and Computer Science, CRHMR co-director Christos Boutopoulos, and the University of Montreal first published this method in the journal Nature. Scientific report In 2020.
Orimi co-wrote with the main authors Katiane Roversi, Sébastien Talbot, Boutopoulos (UdeM), Marcelo Falchetti and Edroaldo da Rocha (University of Santa Catarina, Brazil). The researchers there demonstrated that this technology can be used to properly print sensations. Neuron, an important part of the peripheral nervous system. They say this holds promise for the long-term development of bioprinting potential, including disease modeling. Drug testing and implant manufacturing.
Executable and functional
The researchers tested the technique using dorsal root ganglion (DRG) neurons from the mouse peripheral nervous system. Neurons were suspended in a biological ink solution and loaded into square capillaries above a biocompatible substrate. A low-energy nanosecond laser pulse focused on the center of the capillary, creating microbubbles that expanded and ejected microjets containing cells onto the underlying substrate. The samples were briefly incubated, then washed and reincubated for 48 hours.
The team then performed tests to measure the size of the printed cells. The viability test showed that 86% of the cells were alive 2 days after printing. Researchers say that the lower the energy used by the laser, the better the survival rate. Thermal machines associated with higher use of laser energy were more likely to damage cells.
Other tests measured neurite outgrowth (which produces new projections as developing neurons grow in response to guidance signals), neuropeptide release, calcium imaging, and RNA sequencing. . Overall, the results are generally promising, suggesting that this technology can make a significant contribution to the field of bioprinting.
Good for humans and animals
“In general, when it comes to bioprinting, people often jump to conclusions,” says Orimi. “They think we can now print something like this: Human organs for transplantation. It’s a long-term goal, but we’re a long way from it. However, there are still many ways to use this technology. ”
The closest thing at hand is drug discovery. The team is seeking approval to continue research into cell transplants, which are of great help in the discovery of drugs, such as nerve recovery drugs.
Another advantage of using this technique is that it reduces animal testing. Not only does this have a humanitarian aspect, fewer animals are euthanized for experiments aimed at benefiting humans, but the tests are carried out on human tissue rather than on animals. Therefore, more precise results will be obtained.
For more information:
Hamid Ebrahimi Orimi et al, on-demand cell bioprinting by laser guided lateral transfer (LIST), Scientific report (2020). DOI : 10.1038 / s41598-020-66565-x
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