Bowler Stuart Broad will miss the remainder of the England vs India Test Series with a calf tear.
Broad, 35, was initially excluded from the second test, which begins Thursday, but a scan on Wednesday revealed a tear in her right calf.
He is England’s second-highest wicket-taker and could have played his 150th test at Lord’s.
James Anderson, 39, is also uncertain for Thursday with a quadruple muscle problem – so Saqib Mahmood has been called up.
England, who have Mark Wood and Craig Overton as other bowling options on the squad, expect more information on Anderson’s injury later on Wednesday.
The series is tied at 0-0 after the draw for the first test at Trent Bridge.
Broad, who has 524 wickets, felt a sharp calf pain during a warm-up “shuttle run” during practice in England on Tuesday and immediately left the pitch.
“Things can change so quickly,” Broad wrote on social media. “A moment before training all smiles and then during the warm-up, I jumped a hurdle, I landed a little awkwardly on my right ankle then the next step felt like I had been whipped by a rope as hard as you can imagine on the back of my leg. “
He added: “The season is over and I’m delighted to miss this round of testing in India, but my focus now is Australia. I’m going to take my time, no rush, baby steps and be the fittest I can go there. Great concentration in my brain. ”
England’s fast bowling resources are already exhausted.
Jofra Archer, with an elbow injury, and Olly Stone, with a back problem, are long gone, while Chris Woakes is out with a heel problem.
Versatile Ben Stokes has also taken an indefinite hiatus from cricket to focus on his mental well-being.
Anderson rose to 621 wickets – third on the all-time list – in the first test, but missed practice on Wednesday.
When asked if England could do without their two attacking bosses, batsman Jonny Bairstow told BBC Sport: “I see no reason why we can’t cope.
“We’ve had a lot of people standing up before. We don’t know exactly what will happen tomorrow, but there might be an opportunity for someone to play a test at Lord’s, and that’s an exciting prospect. “
Bairstow admitted it’s “naturally” a blow to lose bowlers of Anderson and Broad’s caliber, but added, “I don’t think it’s too unsettling.
“It’s the nature of professional sport. Sometimes there will be injuries and illnesses. You have to be adaptable. “
England looked set to make changes on their side already, with versatile bowler Moeen Ali likely to replace a batsman to give more depth and variety to the attack.
Drummers Haseeb Hameed and Ollie Pope are also in the running to return.
India will be without fast pitcher Shardul Thakur, who suffers from a hamstring injury.
His absence could mean a return for non-turner Ravichandran Ashwin, who was left out of the first test despite having 413 test wickets under his belt as India opted for four front row bowlers.
“Four fast bowlers means you can apply pressure in every session,” said captain Virat Kohli. “We like to play with this model. “
Kohli has confirmed Thakur will likely be fit for the third test at Headingley.