Bradford City and Lincoln City also form the rest of the United squad, while Liverpool’s Under-23s face Bolton Wanderers, Port Vale and Rochdale.
Arsenal youngsters face Swindon, Newport and Plymouth, while Tottenham face Oxford, Cambridge and Stevenage.
The Chelsea children face Exeter, Bristol Rovers and Cheltenham, and Manchester City face Rotherham United, Doncaster and Scunthorpe.
Sunderland are the current holders of the competition after beating Tranmere on penalties at Wembley in March, a day after the delayed 2019-2020 final when Salford City beat Portsmouth on penalties.
Here are the groups in full:
Everton, Carlisle United, Hartlepool United, Morecambe
Leeds, Oldham Athletic, Tranmere Rovers, Salford City
Wolves, Wigan Athletic, Crewe Alexandra, Town of Shrewsbury
Liverpool, Port Vale, Rochdale, Bolton Wanderers
Manchester City, Rotherham United, Doncaster, Scunthorpe
Manchester United, Bradford, Lincoln, Sunderland
Leicester, Accrington Stanley, Barrow, Fleetwood
Newcastle, Sheffield mercredi, Harrogate, Mansfield
West Ham, Colchester, Gillingham, Ipswich
Crystal Palace, Wimbledon, Portsmouth, Sutton
Aston Villa, MK Dons, Burton, Wycombe
Brighton, vert forêt, Northampton, Walsall
Chelsea, Exeter, Bristol Rovers, Cheltenham
Arsenal, Swindon, Newport, Plymouth
Southampton, Crawley, Charlton, Leyton-Orient
Tottenham, Oxford, Cambridge, Stevenage