With no fan zones available due to COVID-19 restrictions, the city’s mayor, Sadiq Khan, urged supporters not to travel without a ticket or a “safe place” to watch the game.
Trafalgar Square, a traditional tartan army haunt on football trips to London, has been made available for key workers to watch the game.
And with just 3,000 tickets to the game, many will have to try to find a place to watch the action on TV.
England enter the game knowing they will advance to the round of 16 if they beat Scotland after their first victory against Croatia.
Gareth Southgate’s side will be confirmed in the top two if they win and Croatia fail to beat the Czechs earlier today.
Meanwhile, Scotland will look to bounce back from its opening loss to Czech Republic in what was their first game in a major final in 23 years.
Expectation grew among fans on both sides ahead of the second major tournament meeting of international football’s longest-standing rivals.
Southgate started the other goal as Paul Gascoigne’s superb goal closed a 2-0 win for the Wembley group at Euro 96.
Sky News spoke to Scottish fans at the Piper’s Rest pub near Edinburgh Waverley Station before their trip south – all had match tickets.
Martin Riddell said: “We have been following Scotland from near and far and for 23 years and we haven’t had much joy so for us it’s the chance to play in a tournament and we have to. Be there.
“The Scotland-England game moves the hairs on the back of your neck, especially once you’re in this stadium.
“We’ve been through a lot, as Scottish fans, and we deserve our day in the sun… or our night in the rain. “
And although COVID restrictions have put the brakes on the occasion, an estimated 14.8 million pints will be sold across England and Scotland on match day, with more than three million sold during the match. himself.
However, the British Beer and Pub Association (BBPA) has warned that due to restrictions including social distancing, the rule of six and the ban on standing, beer sales will be cut by nearly 850,000. pints.
This would result in a loss of revenue of around £ 3.2million for pubs in England and Scotland.
On the pitch, Southgate has confirmed Harry Maguire will be involved and may even start after recovering from an injury he sustained towards the end of the domestic season.
And Scotland got a boost after Kieran Tierney was declared fit after missing Group D’s opener with a problem.