All 153 employees resigned during the two-week suspension period that began June 8 or were laid off on Tuesday, according to Gale Smith.
Employees who complied with the mandatory vaccination policy during the suspension period returned to work the day after they were brought into compliance, Smith told CNN in an email Tuesday night.
On March 31, Houston Methodist became the first major healthcare system in the country to mandate Covid-19 vaccinations, starting with managers, according to an initial announcement by Houston Methodist CEO Marc Boom.
More than 100 of the hospital workers involved in the trial said the vaccines were “experimental and dangerous” and that it would be “wrong” to be fired for refusing to be vaccinated.
All three vaccines used in the United States currently have emergency use approval from the United States Food and Drug Administration, but not yet full approval from the FDA.
Employees were to be vaccinated against Covid-19 by June 7, CNN previously reported.
Houston Methodist said it did not have information on the number of employees resigning and the number of layoffs.
Boom said earlier this month that 24,947 employees were fully vaccinated and the hospital had reached near full compliance with the mandate, with fewer than 200 suspended for not yet complying.
The Federal Commission for Equal Employment Opportunities said in December that companies can legally require all employees returning to the workplace and new hires to be vaccinated against Covid-19. The only exceptions allowed are for disabilities and religious reasons.
CNN’s Theresa Waldrop contributed to this report.