The retail giant, which has stores in Exeter, Torquay and Plymouth, reopened 97 branches in April as the lockdown in England eased.
However, the decision to welcome customers again was part of an inventory reorientation offer before the stores closed permanently.
There is a huge closing sale with up to 80 percent off products.
The latest round of closures will take place on Saturday May 8 in Derby, Crawley and Torquay, and stores in Exeter and Plymouth will close on Saturday May 15.
Twenty-seven Debenhams stores will close earlier, of which 16 will close on May 2, including branches in Carmarthen, Newbury and Taunton.
Debenhams has confirmed when 27 of its stores will close permanently.
The closing dates for the other branches have not yet been confirmed, but all stores will be closed permanently by May 15.
Stores closing on May 2:
- Is it worth it
- York Monks Cross
Stores close on May 4:
Stores closing on May 8:
- Bury St Edmunds
- Hemel Hempstead
- Leeds city center
- Westwood Cross
Closing of stores before May 15:
- Cheshire Oaks
- Edge of the lake
- Centre Trafford
- White Rose
The second wave of closures will see stores in Bedford, Hastings and Nottingham cease operations on May 4.
The exact closing date for the remaining 45 stores has yet to be decided as it depends on inventory clearance, but Debenhams told The Sun they would all be closed by May 15.
Earlier this year, the 243-year-old retailer confirmed it would not reopen its 15 stores in Scotland following the coronavirus lockdown.
Prices have already been slashed by up to 70% in-store and online, but the retailer will cut them even further in stores that close in early May.
Shoppers will be able to take discounts of up to 80% in the fashion and home departments, while the price of beauty and perfumes will be reduced by up to 70%.
A spokesperson for Debenhams said: “Our stock is flying off the shelves and it’s no surprise these great bargains are available.
“Time is running out for shoppers to take advantage of the incredible discounts offered at these 25 stores.”
The retail chain was bought by Boohoo in January this year in a £ 55million deal that only included the retailer’s name and website.
The 118 physical Debenhams stores and their 12,000 employees were not part of the deal putting thousands of jobs at risk.
All stores were forced to close temporarily when the UK was plunged into a third lockdown in January.
Twenty-one branches never reopened after the lockdown restrictions were eased.
Stores open for sales have implemented Covid security measures to ensure the safety of staff and customers while shopping.
This includes social distancing measures and additional hand sanitizing stations to help stop the spread of the coronavirus.
Savvy buyers are already showing off their Debenhams closing sales, including one who pocketed £ 118 of wetsuits for £ 4.