The boos were clearly audible from the stands at Cambridge Abbey Stadium, but the other spectators were quick to respond by clapping before the start of the Ligue 2 game.
The incident followed an incident similar to Millwall earlier this month.
Sports stars, government ministers and club leaders condemned Millwall supporters, with bosses saying they were ‘dismayed and saddened’, when crowds booed players for hitting their knees ahead of Derby game December 5.
Colchester’s League 2 game with Grimsby on the same day was preceded by fans booing the players’ anti-racism gesture ahead of kick-off.
Essex club owner Robbie Cowling later said fans who want to boo players by taking the knee should not be attending matches.
He said, “Those who take the knee and support the knee grip not only show their willingness to support the drive to eradicate racial oppression, but force it to be a topic of discussion even when it is uncomfortable.
“Maybe those who booed on Saturday could now understand what this gesture means for our club and will at the very least remain silent in the next games while the players continue to take the knee before each kick-off.
“Alternatively, they should just stay away from our club because anyone who still wants to boo now that I’ve explained the purpose and importance of the knee grip is not welcome at our club.
“I’ll be happy to reimburse anyone for the remaining value of their season permit if that’s the reason they feel they can’t attend our games. ”