The Red Roses face Les Bleues in the second of a two-test series on Saturday, November 21 (12:00 PM KO, live on BBC Two and BBC iPlayer).
The Lagi Tuima Center makes its first appearance since June 2019 while Claudia MacDonald takes her place on the wing.
Harriet-Millar Mills starts off on the blind side flanker with Worcester Warriors forward Alex Matthews on the openide.
Ellena Perry, who is returning to Gloucester-Hartpury, is among the finalists.
The final test of the year comes too early for Sarah Hunter who continues her return to the game.
Middleton said: “Playing three physical games against the best opposition in a row is very attractive and can naturally take its toll.
“When I asked Katy if she would be captain of the team I honestly had no idea it would fall on such an occasion and knowing her as I arrived I’m pretty sure she didn’t have it either more. What I do know is that even after winning the World Cup as captain in 2014, the smile on his face when I asked him to become the team captain said all you need to know about one of England’s greatest players.
“Last week we had to see how we’re doing without Katy’s half-flight experience, this week is a chance to see how we’re doing without Emily Scarratt in the starting XV. Lagi has been away for a long time, now she’s back for the Harlequins and training with us and we can’t wait to see what she can do on the Twickenham stage.
“Harriet Millar-Mills really deserves her departure. She was exceptional for us on and off the field.
“We are delighted to welcome Ellena Perry back to the team. She is a player who has quality all over her and someone to whom we attach a lot of importance.
“We are absolutely empty of Sarah [Hunter] but know that we have to manage his return to play appropriately. There’s a big picture over the next 12 months, in September 2021 we’re going to need our captain and that’s our strategy with her.
“Marlie Packer and Sarah Beckett have been tremendous performers for us the past two games and we’re confident we know what they can both do.
“Helena Rowland took a hit against France and hasn’t fully trained this week, but did more than enough last week to keep her place in the squad. It was one of the most composed and impactful early starts I’ve seen from an English player.
“All the players and staff are looking forward to being on Saturday in what we know to be another tough game. As a team and a group of staff, we would like to say a huge thank you to all of the RFU family who made this game possible, to all the supporters who wished us good luck over the past few weeks and to everyone. for their agreement. watch.
“We have decided to work hard and never give our best in this series of games.
“We really want our performances to reflect the effort that so many people have shown and continue to show to keep our game on the pitch during such a difficult time.
“We wanted to put smiles on people’s faces and will give everything we have on Saturday to try to keep people smiling.”
15. Ellie Kildunne (Wasps FC Ladies, 10 sélections)
14. Claudia MacDonald (Wasps FC Ladies, 11 sélections)
13. Lagi Tuima (Harlequins women, 5 caps)
12. Zoe Harrison (Saracens Women, 26 caps)
11. Abby Dow (Wasps FC Ladies, 14 sélections)
10. Katy Daley-Mclean (C; Sale Sharks Women, 115 sélections)
9. Leanne Riley (Harlequins Women, 39 caps)
1. Vickii Cornborough (Harlequins Women, 55 caps)
2. Lark Davies (Loughborough Lightning, 27 sélections)
3. Shaunagh Brown (Harlequins Women, 19 sélections)
4. Abbie Ward (VC; Harlequins Women, 44 sélections
5. Morwenna Talling (Loughborough Lightning, 2 sélections)
6. Harriet Millar-Mills (Wasps FC Ladies, 57 sélections)
7. Alex Matthews (Worcester Warriors, 39 caps)
8. Poppy Cleall (Saracens Women, 42 sélections)
16. Amy Cokayne (Harlequins Women, 52 sélections)
17. Ellena Perry (Gloucester-Hartpury Women, 9 caps)
18. Laura Keates (Worcester Warriors, 61 caps)
19. Sarah Beckett (Harlequins Women, 19 sélections)
20. Marlie Packer (Saracens Women, 73 caps)
21. Natasha Hunt (Gloucester-Hartpury Women, 54 caps)
22. Helena Rowland (Loughborough Lightning, 2 sélections)
23. Emily Scarratt (Loughborough Lightning, 91 sélections)
NPR: Heather Kerr (Wasps FC Ladies, 23 sélections)