It emerged on Tuesday that plans for a European Premier League involving top Premier League clubs had been revived.
The proposed new £ 4.6 billion tournament, which would replace the Champions League, is backed by Fifa, but UEFA says it “strongly opposes” the plans.
Liverpool and Manchester United have been approached to join the league, which could potentially also Arsenal, Chelsea and Manchester City.
A UEFA statement said: “The UEFA president has made it clear on several occasions that UEFA is strongly opposed to a Super League.
“The principles of solidarity, promotion, relegation and open leagues are not negotiable. This is what makes European football work and what makes the Champions League the best sports competition in the world.
“UEFA and the clubs are committed to building on such a force not to destroy it to create a Super League of 10, 12 or even 24 clubs, which would inevitably become boring. “