Four other cases were discovered in wild boars according to the German Ministry of Agriculture. The latest infections were recorded in Brandenburg, a state in eastern Germany.
All German cases so far have been in wild animals, but there are fears that this will spill over into commercially farmed pigs.
According to the Agriculture Ministry, the disease, which is highly contagious, has likely spread to more wild boars.
After the initial outbreak was confirmed in September, China banned pork imports from Germany.
This caused Chinese pork prices to rise and forced German farmers to turn to alternative markets.
African swine fever first appeared in the early 20th century with the first case identified retrospectively in 1907.
It was first identified positively in 1921 after an epidemic in Kenya, then under British colonial control.
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There is no commercially available vaccine against African swine fever.
It comes as the world continues to grapple with the coronavirus epidemic that erupted in China late last year.
The disease has already killed more than a million people and forced countries around the world to self-isolate.
The United States has reported the most deaths and cases of coronavirus, with the infection killing 216,000 people in America.