The 30-year-old returned to the Lightning roster for the first time since February in Game 3 and scored one goal, but had just five teams, playing just 2:47.
Stamkos skated cautiously at the end of his last shift in the first period and was not on the bench for the start of the second. He returned to the bench and did a few laps to test the injury but did not return and was deemed “unfit to play” for Game 4.
Stamkos, who last performed on February 25, underwent surgery in early March and worsened the injury at least once over the summer.
Tampa Bay leads the series 3-2.