The leader of the Spanish league, Barcelona was held to a goalless draw in Seville, leaving the title race wide open with eight games to play.
The result means that Real Madrid, in second, could win points with Barcelona during his move to Real Sociedad on Sunday.
The free kick of Lionel Messi grazed the bar in the first half but Barcelona was frustrated by the defence of Seville.
Lucas Ocampos has also hit the crossbar for the hosts before the break.
Sevilla sat deep, often playing with five at the back, and it has proven to be effective because Barcelona has seen a lot of ball but didn’t create many opportunities clear.
The free-kick from Messi was closest, and the first strike by Luis Suarez has almost crept a few minutes before the full time, but Seville has had flashes of promise attack – Ocampos and Munir El Haddadi forcing two stops intelligent Ter Stegen at a short distance in the first half.
The attacker of Barcelona, Antoine Griezmann came in the game in the 76th minute to try to find a winner but had no impact.
The defender of Real Madrid on loan, Sergio Reguilon, had a great scoring chance for Sevilla in stoppage time when the ball fell kindly for him in a position unmarked in the area, but it hit straight to Ter Stegen.
The league leaders will play next to Athletic Bilbao on Tuesday, 23 June, while Messi remains on 700 career goals.