While six players have agreed to short-term contracts to stay at the club, this represents a significant blow for the Tigers, especially given that Barnsley has also refused a request for an extension of the loan agreement Mallik Wilks.
This means that Wilks will return to his parent club at the end of the month.
The former winger for Leeds United, Wilks, has moved to Barnsley this summer, but made only 15 league appearances, scoring once, before being loaned to Hull in January.
He found more success in the Tigers, scoring three goals in seven league matches.
However, with the form of disastrous Hull the applicant directly in a relegation battle with Barnsley, the Tykes have rejected an offer to extend the loan of winger.
This means that it will only be available for the relegation of Saturday, six points against Charlton Athletic and a trip to Birmingham City before his return to Oakwell.
However, Hull has been boosted by six players accepting short-term contracts to stay with the club.
Jon Toral, Kevin Stewart, Dan Batty, Angus MacDonald, Robbie McKenzie and Will Mannion will remain with the Tigers for nine games remaining of the season at least.
The trio of loan Josh Bowler, Matthew Pennington and Herbie Kane have also extended their temporary stay at the club.
However, the signature of Maddison in January, as well as the defenders Lichaj, Jackson Irvine and Stephen Kingsley have all refused the offer of staying.
The boss of Hull, Grant McCann, has said that the reason why the four players were from was due to the fact that they would not participate in the season.
He said: “It is always a difficult decision to let the guys who have helped the club on and off the field, but all the decisions are taken in the best interest of the football club.
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“I would like to take this opportunity to thank all the players who leave us for the contributions that they have made at Hull City and we wish them all the best for the future.
“In the end, at this stage of the season, we only want players fully committed to the cause of the club and our goal is firmly to work with the group that remains in the head of the last nine games. ”
“The confidence in the camp is clear for all, since we are back to training and everyone is looking forward to resume the combat against Charlton this weekend.”