The rate of confirmed cases in the city today (Wednesday, April 1) is 541– against 451 yesterday.
Another person died at Sheffield hospital after being tested positive for coronavirus, according to the figures, bringing the death toll to 21 in the city.
Elsewhere in South Yorkshire, there are now 122 confirmed cases at Barnsley, 107 at Rotherham and 79 at Doncaster.
The latest figure was provided by Public Health England as part of their daily update for each area.
Sheffield is the most affected location in the UK outside of London and the south of England – the current confirmed total is behind only Birmingham, Hampshire and Surrey by higher level local authorities.
Data for London, which has the highest number of confirmed cases overall, is calculated by each district.
Across the UK, 29,474 tested positive for COVID-19. A total of 2,352 people tested positive unfortunately died.
Meanwhile, the youth were warned not to underestimate the potential impact of the disease after the death of a 13-year-old boy in Brixton, London.
A drug that could help treat the coronavirus is also to be tested in patients in hospitals across England and Scotland, including the teaching hospitals in Sheffield.
Remdesivir trials have been accelerated and will take place at 15 NHS centers.